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M94A0740.TXT
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1994-10-21
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Document 0740
DOCN M94A0740
TI Mechanism of action of Coumermycin A1.
DT 9412
AU Jardine D; Locarnini S; Tachedjian G; Birch C; Virology Dept., Fairfield
Hospital, Victoria.
SO Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:102 (poster no. 54).
Unique Identifier : AIDSLINE ASHM5/94348915
AB AIM: To investigate the mechanism of action of Coumermycin A1 against
HIV. METHODS: We investigated the in vitro anti HIV activity of
coumermycin A1 in acutely infected PBLs, T-cell lines (MT-2) and
macrophages and in chronically infected cells (H9-HTLVIIIB and
macrophages). Several in vitro assays, such as reverse transcription and
CD4/gp120-interaction, were used in an attempt to elucidate the specific
mechanism of action of the drug in the acutely infected lines. In
addition a Coumermycin resistant isolate was generated by passage of the
virus in increasing concentrations of the drug. RESULTS: Coumermycin A1
was active in cells acutely infected with HIV, with complete inhibition
of the virus at 5 micrograms/ml. No inhibition of viral replication was
observed in chronically infected cells. In vitro assays failed to locate
the target of coumermycin in the replication cycle of HIV. Coumermycin
resistant strains of HIV have been generated. CONCLUSION: Because
Coumermycin inhibits HIV replication in acute but not chronic infections
suggests that its target is at or before integration. However in vitro
assays targeting attachment, penetration and reverse transcription have
ruled out these processes as targets.
DE Antibiotics/PHARMACOLOGY Cell Line Coumarins/PHARMACOLOGY Human
HIV/*DRUG EFFECTS In Vitro Macrophages/MICROBIOLOGY
T-Lymphocytes/MICROBIOLOGY Virus Integration/DRUG EFFECTS Virus
Replication/*DRUG EFFECTS MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).